Pair Name	Thymoquinone, Methotrexate
Disease Info	[ICD-11: 2B51]	Osteosarcoma		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Up-regulation	Expression	CASP3	hsa836
	Down-regulation	Expression	BCL2	hsa596
In Vitro Model	SaOS-2	Osteosarcoma	Homo sapiens (Human)	CVCL_0548
Result	The findings of the present study highlight new insights into understanding the role of TQ as a potential therapeutic agent in osteosarcoma by increasing MTX-induced apoptosis.

Pair Name	Thymoquinone, Methotrexate
Disease Info	[ICD-11: 2B51]	Osteosarcoma		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Up-regulation	Expression	BAX	hsa581
	Up-regulation	Expression	CASP9	hsa842
	Down-regulation	Expression	BCL2	hsa596
In Vitro Model	SaOS-2	Osteosarcoma	Homo sapiens (Human)	CVCL_0548
Result	The co-treatment of TQ and MTX is associated with the up-regulation of apoptotic factors and down-regulation of anti-apoptotic factors, conducting apoptosis aggravation and OS cell death.